Search results for "HUMAN HEMOFILTRATE"

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Automated multi-dimensional liquid chromatography

2004

A comprehensive on-line sample clean-up with an integrated two-dimensional HPLC system was developed for the analysis of natural peptides. Samples comprised of endogenous peptides with molecular weights up to 20 kDa were generated from human hemofiltrate (HF) obtained from patients with chronic renal failure. The (poly-)peptides were separated using novel silica-based restricted access materials with strong cation-exchange functionalities (SCX-RAM). The size-selective sample fractionation step is followed by cation-exchange chromatography as the first dimension. The subsequent second dimension of separation is based on hydrophobic interaction using four parallel short reversed-phase (RP) co…

PROTEINSClinical BiochemistryMolecular Sequence DataAnalytical chemistryMass spectrometryBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryCIRCULATING HUMAN PEPTIDESColumn chromatographyHumansSample preparationhuman blood filtrateAmino Acid SequenceHUMAN PLASMAPeptide sequenceChromatography High Pressure LiquidChromatographyEdman degradationMolecular masssample preparationChemistryMIXTURESCell BiologyGeneral MedicineReversed-phase chromatographyMASS-SPECTROMETRYENDOSTATINChromatography Ion ExchangeHUMAN HEMOFILTRATEpeptidesSEPARATIONidentificationHPLCFiltrationJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

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PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
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  - PE2_3Experimental particle physics with accelerators
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  - PE2_6Nuclear, hadron and heavy ion physics
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  - PE2_11Ultra-cold atoms and molecules
  - PE2_18Equilibrium and non-equilibrium statistical mechanics: steady states and dynamics
  - PE2_14Lasers, ultra-short lasers and laser physics
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  - PE2_16Non-linear physics
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  - PE2_13Quantum optics and quantum information
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- PE1Mathematics
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LSLife Sciences
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  - LS1_14Innovative methods and modelling in molecular, structural and synthetic biology
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- LS2Integrative Biology: from Genes and Genomes to Systems
  - LS2_1Genetics
  - LS2_3Epigenetics
  - LS2_2Gene editing
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  - LS2_9Metabolomics
  - LS2_10Glycomics/Lipidomics
  - LS2_11Bioinformatics and computational biology
  - LS2_12Biostatistics
  - LS2_14Genetic diseases
  - LS2_16Innovative methods and modelling in integrative biology
  - LS2_13Systems biology
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- LS4Physiology in Health, Disease and Ageing
  - LS4_1Organ and tissue physiology and pathophysiology
  - LS4_4Endocrinology
  - LS4_3Physiology of ageing
  - LS4_2Comparative physiology
  - LS4_5Non-hormonal mechanisms of inter-organ and tissue communication
  - LS4_6Microbiome and host physiology
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- LS3Cell Biology, Development, Stem Cells and Regeneration
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  - LS3_7Mechanobiology of cells, tissues and organs
  - LS3_8Embryogenesis, pattern formation, morphogenesis
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- LS5Neuroscience and Disorders of the Nervous System
  - LS5_1Neuronal cells
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- LS9Biotechnology and Biosystems Engineering
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  - LS9_5Food biotechnology and bioengineering
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  - LS9_7Environmental biotechnology and bioengineering
  - LS9_8Applied plant sciences, plant breeding, agroecology and soil biology
  - LS9_10Veterinary and applied animal sciences
  - LS9_9Plant pathology and pest resistance
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  - LS9_1Bioengineering for synthetic and chemical biology
SHSocial Sciences and Humanities
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- SH6The Study of the Human Past
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  - SH5_2Theory and history of literature, comparative literature
- SH7Human Mobility, Environment, and Space
  - SH7_2Migration
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- SH8Studies of Cultures and Arts
  - SH8_1Kinship; diversity and identities, gender, interethnic relations
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  - SH8_8Visual and performing arts, screen, arts-based research
  - SH8_9Digital approaches to anthropology, cultural studies and art